In order to understand their functional roles, we downregulate the candidate genes in different cell lines and verify it with Western Blot by using specific antibodies. The main purpose of our work is to identify new functional roles of candidate genes in the cellular network. The stable and ubiquitous expression of GAPDH also make it a suitable loading control for many experiments. Browse Similar Products: Beta-Actin Antibodies. GAPDH (36 kDa) is integral for glycolysis and plays many roles in nuclear function such as transcription regulation and apoptosis. However, the 41 kDa cleaved fragment of beta. Novus offers a complete selection of highly characterized beta-Actin antibodies. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. The expression levels of this protein do not vary drastically due to cellular treatment, which is another reason the protein makes a suitable control. Western blot analysis using anti-Beta Actin mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysates.īeta-Actin (42 kDa) is commonly chosen as a loading control due to its general expression across all eukaryotic cell types. Western blot analysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.īeta-Actin Antibody (8H10D10) This Anti-beta-Actin Antibody, clone 4C2 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin. Beta-actin is the most commonly used loading control for western blot (see Labomes dedicated discussion on loading control for western blot). The most popular loading control targets include Beta-Actin and GAPDH. Additionally, the loading control protein should demonstrate ubiquitous expression. When considering a loading control for your experiment, it is important to choose a protein that does not have a similar molecular weight to your target of interest. A loading control antibody helps determine if samples have been loaded equally across all wells and confirms effective protein transfer during the western blot protocol. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. In higher eukaryotes, there are six isoforms of actin.Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. compared with normal ovarian tissues, as measured by Lectin chip, Western blot and Lectin blot analyses, as well as ovarian tissue microarray analysis. Actin is expressed in all eukaryotic cells. It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells. In PBS containing 0.02% sodium azide.Īctin is one of the major proteins of the cytoskeleton. Optimal conditions must be determined individually for each application.ĭetects a band of ~43kDa by Western blot. Suggested dilutions/conditions may not be available for all applications. Western blot analysis of recombinant Actin isoforms using -Actin (13E5) Rabbit mAb (upper) and Pan-Actin Antibody 4968 (lower). Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Please note this antibody is raised against the b-nonmuscle. Beta and gamma 1 are two non-muscle actin proteins. Synthetic peptide corresponding to the sequence near the C-terminus of human β-Actin. This antibody detects a single clean band at just above 40kD that corresponds to beta Actin.
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